TY - GEN
T1 - A qcm-based lab-on-a-chip device for real time characterization of shearinduced platelets adhesion and aggregation
AU - Sun, Hongwei
AU - Wang, Pengtao
AU - Liu, Moli
AU - Xu, Jin
PY - 2012
Y1 - 2012
N2 - The long-term goal of this project is to develop a microfluidic device integrated with a quartz crystal microbalance (QCM) sensor to perform real-time monitoring of platelet adhesion and aggregation under various hemodynamic conditions. This Lab- On-a-Chip device was fabricated with softlithography technique and plasma bonding. The gold sensing surface (electrode) of QCM sensor was embedded in the sensing area of microchannel, in which different fluid solutions were driven through to induce required shear flows for protein interaction study. The time-dependent (transient) frequency shift upon flowing blood samples was monitored to characterize the dynamic process of the platelet adhesion and protein interaction. The interaction between recombinant platelet surface receptor glycoprotein Ibα (GPIbα) and von Willebrand factor (vWF) were investigated under both static and dynamic flow conditions. It was found that the association process is much faster than disassociation process. This device functions as a powerful platform for studying the impact of flow pattern and shear stress on platelet function and GPIbα and vWF interaction, and potentially serves as a prototype for cardiovascular diagnostic purposes.
AB - The long-term goal of this project is to develop a microfluidic device integrated with a quartz crystal microbalance (QCM) sensor to perform real-time monitoring of platelet adhesion and aggregation under various hemodynamic conditions. This Lab- On-a-Chip device was fabricated with softlithography technique and plasma bonding. The gold sensing surface (electrode) of QCM sensor was embedded in the sensing area of microchannel, in which different fluid solutions were driven through to induce required shear flows for protein interaction study. The time-dependent (transient) frequency shift upon flowing blood samples was monitored to characterize the dynamic process of the platelet adhesion and protein interaction. The interaction between recombinant platelet surface receptor glycoprotein Ibα (GPIbα) and von Willebrand factor (vWF) were investigated under both static and dynamic flow conditions. It was found that the association process is much faster than disassociation process. This device functions as a powerful platform for studying the impact of flow pattern and shear stress on platelet function and GPIbα and vWF interaction, and potentially serves as a prototype for cardiovascular diagnostic purposes.
UR - http://www.scopus.com/inward/record.url?scp=84882362757&partnerID=8YFLogxK
U2 - 10.1115/ICNMM2012-73205
DO - 10.1115/ICNMM2012-73205
M3 - Conference contribution
AN - SCOPUS:84882362757
SN - 9780791844793
T3 - ASME 2012 10th Int. Conf. on Nanochannels, Microchannels, and Minichannels Collocated with the ASME 2012 Heat Transfer Summer Conf. and the ASME 2012 Fluids Engineering Division Sum, ICNMM 2012
SP - 17
EP - 22
BT - ASME 2012 10th Int. Conf. on Nanochannels, Microchannels, and Minichannels Collocated with the ASME 2012 Heat Transfer Summer Conference and the ASME 2012 Fluids Engineering Division Sum, ICNMM 2012
T2 - ASME 2012 10th Int. Conf. on Nanochannels, Microchannels, and Minichannels, ICNMM 2012 Collocated with the ASME 2012 Heat Transfer Summer Conf. and the ASME 2012 Fluids Engineering Division Sum, ICNMM 2012
Y2 - 8 July 2012 through 12 July 2012
ER -