Abstract
The sensitivity of Neutron Macromolecular Crystallography to the presence of hydrogen makes it a powerful tool to complement X-ray crystallographic studies using protein crystals. The power of this technique is currently limited by the relative low neutron flux provided by even the most powerful neutron sources. The strong polarization dependence of the neutron scattering cross section of hydrogen will allow us to use Dynamic Nuclear Polarization to dramatically improve the signal to noise ratio of neutron diffraction data, delivering order of magnitude gains in performance, and enabling measurements of radically smaller crystals of larger protein systems than are possible today. We present a prototype frozen spin system, built at Oak Ridge National Laboratory to polarize single protein crystals on the IMAGINE beamline at the High Flux Isotope Reactor (HFIR). Details of the design and construction will be described, as will the performance of the system offline and during preliminary tests at HFIR.
| Original language | English |
|---|---|
| Pages (from-to) | 430-434 |
| Number of pages | 5 |
| Journal | Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment |
| Volume | 940 |
| DOIs | |
| State | Published - Oct 1 2019 |
Funding
Research sponsored by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory , managed by UT-Battelle, LLC, for the U. S. Department of Energy. The construction and installation of the IMAGINE beam line was partly supported by NSF grant CHE-0922719 . This manuscript has been authored in part by UT-Battelle, LLC, under contract DE-AC05-00OR22725 with the US Department of Energy (DOE). The US government retains and the publisher, by accepting the article for publication, acknowledges that the US government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript, or allow others to do so, for US government purposes. DOE will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).The authors would like to acknowledge the support provided by the HFIR in the installation of the DNP system on the IMAGINE beam-line. The Neutron Sciences Directorate Detector group provided the prototype detector and assisted with the data acquisition. The Jefferson Lab Target Group offered insight into the design of the sample space. The authors also would like to acknowledge the help of all of the IMAGINE instrument team. Research sponsored by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory, managed by UT-Battelle, LLC, for the U. S. Department of Energy. The construction and installation of the IMAGINE beam line was partly supported by NSF grant CHE-0922719. ☆ This manuscript has been authored in part by UT-Battelle, LLC, under contract DE-AC05-00OR22725 with the US Department of Energy (DOE). The US government retains and the publisher, by accepting the article for publication, acknowledges that the US government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript, or allow others to do so, for US government purposes. DOE will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).
| Funders | Funder number |
|---|---|
| U. S. Department of Energy | |
| National Science Foundation | CHE-0922719 |
| U.S. Department of Energy | DE-AC05-00OR22725 |
| National Sleep Foundation | |
| Oak Ridge National Laboratory |
Keywords
- Dynamic nuclear polarization
- Neutron scattering
- Polarized target
- Protein crystallography