A novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens

M. Le Puil, J. P. Biggerstaff, B. L. Weidow, J. R. Price, S. A. Naser, D. C. White, R. S. Alberte

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A novel fluorescence imaging technique based on deconvolution microscopy and spectral analysis is presented here as an alternative to confocal laser scanning microscopy. It allowed rapid, specific and simultaneous identification of five major opportunistic pathogens, relevant for public health, in suspension and provided quantitative results.

Original languageEnglish
Pages (from-to)597-602
Number of pages6
JournalJournal of Microbiological Methods
Volume67
Issue number3
DOIs
StatePublished - Dec 2006

Keywords

  • Confocal microscopy
  • Deconvolution
  • FISH
  • Pathogens
  • Spectral imaging

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