Abstract
The conjugate of antisense c-raf oligonucleotide (ODN) and poly(ethylene glycol) (PEG) was synthesized for intracellular ODN delivery. When combined with polyethylenimine (PEI), the ODN-PEG conjugate self-associated to form polyelectrolyte complex micelles in aqueous solution. The effective hydrodynamic diameter of the micelles was ca. 70 nm with a narrow size distribution. Flow cytometry analysis indicated that the cellular uptake of the micelles by A2780 cells was much higher than that of ODN alone. The micelles also showed a superior antiproliferative activity against ovarian cancer cells in vitro and in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 183-191 |
| Number of pages | 9 |
| Journal | Journal of Controlled Release |
| Volume | 93 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 5 2003 |
| Externally published | Yes |
Funding
This study was supported from the Ministry of Science and Technology (M10214000117-02b1500-02110), from National Cancer Center (02-3-150), from the Ministry of Information and Communication (IMT-2000-B3-2), Republic of Korea.
Keywords
- Conjugate
- Delivery
- Micelles
- ODN
- PEG
Fingerprint
Dive into the research topics of 'A new antisense oligonucleotide delivery system based on self-assembled ODN-PEG hybrid conjugate micelles'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver