A new antisense oligonucleotide delivery system based on self-assembled ODN-PEG hybrid conjugate micelles

Ji Hoon Jeong; Sung Wan Kim; Tae Gwan Park

doi:10.1016/j.jconrel.2003.07.002

A new antisense oligonucleotide delivery system based on self-assembled ODN-PEG hybrid conjugate micelles

Ji Hoon Jeong, Sung Wan Kim, Tae Gwan Park

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

The conjugate of antisense c-raf oligonucleotide (ODN) and poly(ethylene glycol) (PEG) was synthesized for intracellular ODN delivery. When combined with polyethylenimine (PEI), the ODN-PEG conjugate self-associated to form polyelectrolyte complex micelles in aqueous solution. The effective hydrodynamic diameter of the micelles was ca. 70 nm with a narrow size distribution. Flow cytometry analysis indicated that the cellular uptake of the micelles by A2780 cells was much higher than that of ODN alone. The micelles also showed a superior antiproliferative activity against ovarian cancer cells in vitro and in vivo.

Original language	English
Pages (from-to)	183-191
Number of pages	9
Journal	Journal of Controlled Release
Volume	93
Issue number	2
DOIs	https://doi.org/10.1016/j.jconrel.2003.07.002
State	Published - Dec 5 2003
Externally published	Yes

Funding

This study was supported from the Ministry of Science and Technology (M10214000117-02b1500-02110), from National Cancer Center (02-3-150), from the Ministry of Information and Communication (IMT-2000-B3-2), Republic of Korea.

Funders	Funder number
National Cancer Center	02-3-150
Ministerio de Ciencia y Tecnología	M10214000117-02b1500-02110
Ministry of Information and Communication	IMT-2000-B3-2

Keywords

Conjugate
Delivery
Micelles
ODN
PEG

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jconrel.2003.07.002

Cite this

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title = "A new antisense oligonucleotide delivery system based on self-assembled ODN-PEG hybrid conjugate micelles",

abstract = "The conjugate of antisense c-raf oligonucleotide (ODN) and poly(ethylene glycol) (PEG) was synthesized for intracellular ODN delivery. When combined with polyethylenimine (PEI), the ODN-PEG conjugate self-associated to form polyelectrolyte complex micelles in aqueous solution. The effective hydrodynamic diameter of the micelles was ca. 70 nm with a narrow size distribution. Flow cytometry analysis indicated that the cellular uptake of the micelles by A2780 cells was much higher than that of ODN alone. The micelles also showed a superior antiproliferative activity against ovarian cancer cells in vitro and in vivo.",

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AU - Jeong, Ji Hoon

AU - Kim, Sung Wan

AU - Park, Tae Gwan

PY - 2003/12/5

Y1 - 2003/12/5

N2 - The conjugate of antisense c-raf oligonucleotide (ODN) and poly(ethylene glycol) (PEG) was synthesized for intracellular ODN delivery. When combined with polyethylenimine (PEI), the ODN-PEG conjugate self-associated to form polyelectrolyte complex micelles in aqueous solution. The effective hydrodynamic diameter of the micelles was ca. 70 nm with a narrow size distribution. Flow cytometry analysis indicated that the cellular uptake of the micelles by A2780 cells was much higher than that of ODN alone. The micelles also showed a superior antiproliferative activity against ovarian cancer cells in vitro and in vivo.

AB - The conjugate of antisense c-raf oligonucleotide (ODN) and poly(ethylene glycol) (PEG) was synthesized for intracellular ODN delivery. When combined with polyethylenimine (PEI), the ODN-PEG conjugate self-associated to form polyelectrolyte complex micelles in aqueous solution. The effective hydrodynamic diameter of the micelles was ca. 70 nm with a narrow size distribution. Flow cytometry analysis indicated that the cellular uptake of the micelles by A2780 cells was much higher than that of ODN alone. The micelles also showed a superior antiproliferative activity against ovarian cancer cells in vitro and in vivo.

KW - Conjugate

KW - Delivery

KW - Micelles

KW - ODN

KW - PEG

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SN - 0168-3659

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JF - Journal of Controlled Release

IS - 2

ER -

A new antisense oligonucleotide delivery system based on self-assembled ODN-PEG hybrid conjugate micelles

Abstract

Funding

Keywords

UN SDGs

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