A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew

Miaomiao Li; Huaizhi Zhang; Huixin Xiao; Keyu Zhu; Wenqi Shi; Dong Zhang; Yong Wang; Lijun Yang; Qiuhong Wu; Jingzhong Xie; Yongxing Chen; Dan Qiu; Guanghao Guo; Ping Lu; Beibei Li; Lei Dong; Wenling Li; Xuejia Cui; Lingchuan Li; Xiubin Tian; Chengguo Yuan; Yiwen Li; Dazhao Yu; Eviatar Nevo; Tzion Fahima; Hongjie Li; Lingli Dong; Yusheng Zhao; Zhiyong Liu

doi:10.1038/s41467-024-47497-w

A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew

Miaomiao Li, Huaizhi Zhang, Huixin Xiao, Keyu Zhu, Wenqi Shi, Dong Zhang, Yong Wang, Lijun Yang, Qiuhong Wu, Jingzhong Xie, Yongxing Chen, Dan Qiu, Guanghao Guo, Ping Lu, Beibei Li, Lei Dong, Wenling Li, Xuejia Cui, Lingchuan Li, Xiubin TianChengguo Yuan, Yiwen Li, Dazhao Yu, Eviatar Nevo, Tzion Fahima, Hongjie Li, Lingli Dong, Yusheng Zhao, Zhiyong Liu

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Abstract

Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance.

Original language	English
Article number	3124
Journal	Nature Communications
Volume	15
Issue number	1
DOIs	https://doi.org/10.1038/s41467-024-47497-w
State	Published - Dec 2024
Externally published	Yes

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Li, M., Zhang, H., Xiao, H., Zhu, K., Shi, W., Zhang, D., Wang, Y., Yang, L., Wu, Q., Xie, J., Chen, Y., Qiu, D., Guo, G., Lu, P., Li, B., Dong, L., Li, W., Cui, X., Li, L., ... Liu, Z. (2024). A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew. Nature Communications, 15(1), Article 3124. https://doi.org/10.1038/s41467-024-47497-w

@article{81ed41f0e1974e838fd96cbafa09068b,

title = "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew",

abstract = "Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance.",

author = "Miaomiao Li and Huaizhi Zhang and Huixin Xiao and Keyu Zhu and Wenqi Shi and Dong Zhang and Yong Wang and Lijun Yang and Qiuhong Wu and Jingzhong Xie and Yongxing Chen and Dan Qiu and Guanghao Guo and Ping Lu and Beibei Li and Lei Dong and Wenling Li and Xuejia Cui and Lingchuan Li and Xiubin Tian and Chengguo Yuan and Yiwen Li and Dazhao Yu and Eviatar Nevo and Tzion Fahima and Hongjie Li and Lingli Dong and Yusheng Zhao and Zhiyong Liu",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",

year = "2024",

month = dec,

doi = "10.1038/s41467-024-47497-w",

language = "English",

volume = "15",

journal = "Nature Communications",

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Li, M, Zhang, H, Xiao, H, Zhu, K, Shi, W, Zhang, D, Wang, Y, Yang, L, Wu, Q, Xie, J, Chen, Y, Qiu, D, Guo, G, Lu, P, Li, B, Dong, L, Li, W, Cui, X, Li, L, Tian, X, Yuan, C, Li, Y, Yu, D, Nevo, E, Fahima, T, Li, H, Dong, L, Zhao, Y & Liu, Z 2024, 'A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew', Nature Communications, vol. 15, no. 1, 3124. https://doi.org/10.1038/s41467-024-47497-w

TY - JOUR

T1 - A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew

AU - Li, Miaomiao

AU - Zhang, Huaizhi

AU - Xiao, Huixin

AU - Zhu, Keyu

AU - Shi, Wenqi

AU - Zhang, Dong

AU - Wang, Yong

AU - Yang, Lijun

AU - Wu, Qiuhong

AU - Xie, Jingzhong

AU - Chen, Yongxing

AU - Qiu, Dan

AU - Guo, Guanghao

AU - Lu, Ping

AU - Li, Beibei

AU - Dong, Lei

AU - Li, Wenling

AU - Cui, Xuejia

AU - Li, Lingchuan

AU - Tian, Xiubin

AU - Yuan, Chengguo

AU - Li, Yiwen

AU - Yu, Dazhao

AU - Nevo, Eviatar

AU - Fahima, Tzion

AU - Li, Hongjie

AU - Dong, Lingli

AU - Zhao, Yusheng

AU - Liu, Zhiyong

PY - 2024/12

Y1 - 2024/12

N2 - Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance.

AB - Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance.

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U2 - 10.1038/s41467-024-47497-w

DO - 10.1038/s41467-024-47497-w

M3 - Article

C2 - 38600164

AN - SCOPUS:85189946280

SN - 2041-1723

VL - 15

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 3124

ER -

A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew

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