A disposable bulk-acoustic-wave microalga trapping device for real-time water monitoring

Quan Yuan; Hadi Mirzajani; Barbara Evans; Elias Greenbaum; Jayne Wu

doi:10.1016/j.snb.2019.127388

A disposable bulk-acoustic-wave microalga trapping device for real-time water monitoring

Quan Yuan, Hadi Mirzajani, Barbara Evans, Elias Greenbaum, Jayne Wu

Geochemistry & Interfacial Science

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Recently, there has been an increase in the use of algal physiology as a convenient and useful indicator for monitoring of water quality in aquatic ecosystems. However, current methods for monitoring algal physiology are high-cost, power intensive, and have limited sensitivity for practical samples in which algal concentration is low. In order to alleviate these problems, we developed a simple-to-fabricate acoustophoretic particle trapping device that can effectively enrich various types of microalgae such as chlorella and diatoms for real-time monitoring of water quality. This microalgae trap is fabricated by embedding a piezoelectric resonator in a single channel. In comparison to previously reported acoustophoretic particle traps, this device has a simple structure and does not require sheath flows, which makes the device low cost and simple to fabricate and operate. Using Chlorella kessleri and the marine diatom Thalassiosira pseudonana as model algae, the microalgae trap has demonstrated notable particle trapping efficiencies between 82 % to 74 % for Chlorella with a through-flow of 0.5 μl/min to 2.7 μl/min and 85 % to 79 % for diatoms with a 0.5 μl/min to 4 μl/min through-flow. The trap has also shown to simultaneously trap Chlorella and diatoms at different heights of the microchannel. This device has high promise for trapping, separating and manipulating microalgae.

Original language	English
Article number	127388
Journal	Sensors and Actuators, B: Chemical
Volume	304
DOIs	https://doi.org/10.1016/j.snb.2019.127388
State	Published - Feb 1 2020

Funding

The following is Supplementary data to this article: Quan Yuan received his Ph.D. in Electrical Engineering from the University of Tennessee, Knoxville (UTK), 2014. He received his Bachelor degree from Changsha University of Science and Technology (CUST), Changsha, China, and Master of Science degree from UTK, both in Electrical Engineering. He is currently a senior research scientist at Weldon School of Biomedical Engineering in Purdue University. His research interests are micro/nano-fluidics based bio-particle manipulation with AC electrokinetics and acoustics, BioMEMS fabrication, system integration with biosensing, optogenetics and microelectronics for Lab-on-a chip application. Hadi Mirzajani received his MSc degree in Electronic Engineering with focus on microelectromechanical systems technology from Sahand University of Technology, Tabriz, Iran. He was a Researcher with Micro Analysis Systems Laboratory, Department of Electrical Engineering and Computer Science, University of Tennessee, Knoxville. Currently, he is an R&TD researcher at Hamava Company, Tehran, Iran. His main research interests include microfluidics, wireless powering of biomedical devices, lab-on-a-chip and MEMS sensors. He has published more than 29 papers in highly ranked journals and conferences. Barbara R. Evans Barbara R. Evans received her graduate degree in chemistry from the University of California San Diego for studies of lamprey transferrin in the laboratory of Prof. Russell Doolittle. During her postdoctoral appointments at Scripps Institute, La Jolla, and the University of Texas at Austin, she studied yeast DNA recombinases in the laboratory of Prof. Makkuni Jayaram. She initially came to Oak Ridge National Laboratory to study lignocellulosic biomass and its degradation by cellulase enzymes. She is continuing work in this area as a member of a multidisciplinary team that is applying integrated characterization, neutron scattering, and computer simulation to investigate the molecular hierarchical structure of plant cell walls. Other research areas that she has studied include bacterial cellulose-based composite materials, plant photosystems, and algal hydrogen production. Elias Greenbaum is President of GTA, Inc., a company whose mission is to expand the offshore wind energy business model by adding renewable hydrogen production (and storage) by subsea electrolyzers that are powered by offshore wind turbines. Greenbaum was appointed co-leader of the DOE H2@Scale CRADA hydrogen production working group. He is a fellow of the American Physical Society and the American Association for the Advancement of Science. He is a former staff member and group leader in the Chemical Technology/Chemical Sciences Divisions at Oak Ridge National Laboratory and received the ORNL 2000 Scientist of the Year award. He is UT-Battelle Corporate Fellow emeritus and UT-Battelle Distinguished Inventor. He received a Ph.D. in nuclear physics and materials science from Columbia University. Jie (Jayne) Wu received the Ph.D. degree in applied physics from the Chinese Academy of Sciences, Beijing, China, in 1999, and the Ph.D. degree in electrical engineering from the University of Notre Dame, Notre Dame, IN, in 2004. From August 2003 to July 2004, Dr. Wu was a Postdoctoral Research Fellow with the Center for Microfluidics and Medical Diagnostics, Department of Chemical and Biomolecular Engineering, University of Notre Dame. Dr. Wu received a US National Science Foundation Career Award in 2005 and Oak Ridge Associated Universities Power Junior Faculty Award in 2006. Currently, she is a Full Professor with the Department of Electrical Engineering and Computer Science, University of Tennessee, Knoxville. Her research interests are solid-state device physics, bio-micro-electromechanical systems, sensors, microfluidics, and lab-on-a-chip. This research was supported by funding from the University of Tennessee Initiative for Pointing Detection and Nanobiosensing, Institute for Secure and Sustainable Environment , and by Oak Ridge National Laboratory’s (ORNL) Technology Transfer and Economic Development Maturation funding program , grant number UTB1120 . Q. Yuan thanks the support of University of Tennessee Professional Development Award . H. Mirzajani thanks the financial support from The University of Tennessee Initiative for Pointing Detection and Nanobiosensing . J . Wu also acknowledges the support of National Natural Science Foundation of China (NNSFC) ( 51728502 ). Oak Ridge National Laboratory is managed by UT-Battelle LLC for the U.S. Department of Energy under contract DE-AC05-00OR22725. Appendix A This research was supported by funding from the University of Tennessee Initiative for Pointing Detection and Nanobiosensing, Institute for Secure and Sustainable Environment, and by Oak Ridge National Laboratory's (ORNL) Technology Transfer and Economic Development Maturation funding program, grant number UTB1120. Q. Yuan thanks the support of University of Tennessee Professional Development Award. H. Mirzajani thanks the financial support from The University of Tennessee Initiative for Pointing Detection and Nanobiosensing. J. Wu also acknowledges the support of National Natural Science Foundation of China (NNSFC) (51728502). Oak Ridge National Laboratory is managed by UT-Battelle LLC for the U.S. Department of Energy under contract DE-AC05-00OR22725.

Funders	Funder number
US National Science Foundation
UT-Battelle LLC
University of Tennessee Initiative for Pointing Detection and Nanobiosensing
University of Tennessee Initiative for Pointing Detection and Nanobiosensing, Institute for Secure and Sustainable Environment
U.S. Department of Energy	DE-AC05-00OR22725
Oak Ridge Associated Universities
Oak Ridge National Laboratory	UTB1120, ORNL
University of Tennessee
UT-Battelle
National Natural Science Foundation of China	51728502, NNSFC

Keywords

Bulk-Acoustic-Wave (BAW)
Low-cost fabrication
Microalgae
Microparticle trapping

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10.1016/j.snb.2019.127388

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abstract = "Recently, there has been an increase in the use of algal physiology as a convenient and useful indicator for monitoring of water quality in aquatic ecosystems. However, current methods for monitoring algal physiology are high-cost, power intensive, and have limited sensitivity for practical samples in which algal concentration is low. In order to alleviate these problems, we developed a simple-to-fabricate acoustophoretic particle trapping device that can effectively enrich various types of microalgae such as chlorella and diatoms for real-time monitoring of water quality. This microalgae trap is fabricated by embedding a piezoelectric resonator in a single channel. In comparison to previously reported acoustophoretic particle traps, this device has a simple structure and does not require sheath flows, which makes the device low cost and simple to fabricate and operate. Using Chlorella kessleri and the marine diatom Thalassiosira pseudonana as model algae, the microalgae trap has demonstrated notable particle trapping efficiencies between 82 % to 74 % for Chlorella with a through-flow of 0.5 μl/min to 2.7 μl/min and 85 % to 79 % for diatoms with a 0.5 μl/min to 4 μl/min through-flow. The trap has also shown to simultaneously trap Chlorella and diatoms at different heights of the microchannel. This device has high promise for trapping, separating and manipulating microalgae.",

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AU - Evans, Barbara

AU - Greenbaum, Elias

AU - Wu, Jayne

PY - 2020/2/1

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N2 - Recently, there has been an increase in the use of algal physiology as a convenient and useful indicator for monitoring of water quality in aquatic ecosystems. However, current methods for monitoring algal physiology are high-cost, power intensive, and have limited sensitivity for practical samples in which algal concentration is low. In order to alleviate these problems, we developed a simple-to-fabricate acoustophoretic particle trapping device that can effectively enrich various types of microalgae such as chlorella and diatoms for real-time monitoring of water quality. This microalgae trap is fabricated by embedding a piezoelectric resonator in a single channel. In comparison to previously reported acoustophoretic particle traps, this device has a simple structure and does not require sheath flows, which makes the device low cost and simple to fabricate and operate. Using Chlorella kessleri and the marine diatom Thalassiosira pseudonana as model algae, the microalgae trap has demonstrated notable particle trapping efficiencies between 82 % to 74 % for Chlorella with a through-flow of 0.5 μl/min to 2.7 μl/min and 85 % to 79 % for diatoms with a 0.5 μl/min to 4 μl/min through-flow. The trap has also shown to simultaneously trap Chlorella and diatoms at different heights of the microchannel. This device has high promise for trapping, separating and manipulating microalgae.

AB - Recently, there has been an increase in the use of algal physiology as a convenient and useful indicator for monitoring of water quality in aquatic ecosystems. However, current methods for monitoring algal physiology are high-cost, power intensive, and have limited sensitivity for practical samples in which algal concentration is low. In order to alleviate these problems, we developed a simple-to-fabricate acoustophoretic particle trapping device that can effectively enrich various types of microalgae such as chlorella and diatoms for real-time monitoring of water quality. This microalgae trap is fabricated by embedding a piezoelectric resonator in a single channel. In comparison to previously reported acoustophoretic particle traps, this device has a simple structure and does not require sheath flows, which makes the device low cost and simple to fabricate and operate. Using Chlorella kessleri and the marine diatom Thalassiosira pseudonana as model algae, the microalgae trap has demonstrated notable particle trapping efficiencies between 82 % to 74 % for Chlorella with a through-flow of 0.5 μl/min to 2.7 μl/min and 85 % to 79 % for diatoms with a 0.5 μl/min to 4 μl/min through-flow. The trap has also shown to simultaneously trap Chlorella and diatoms at different heights of the microchannel. This device has high promise for trapping, separating and manipulating microalgae.

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A disposable bulk-acoustic-wave microalga trapping device for real-time water monitoring

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