TY - JOUR
T1 - A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides
AU - Martin, W. Elliott
AU - Srijanto, Bernadeta R.
AU - Collier, C. Patrick
AU - Vosch, Tom
AU - Richards, Christopher I.
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - The effect of gold and aluminum zero-mode waveguides (ZMWs) on the brightness of immobilized single emitters was characterized by probing fluorophores that absorb in the green and red regions of the visible spectrum. Aluminum ZMWs enhance the emission of Atto565 fluorophores upon green excitation, but they do not enhance the emission of Atto647N fluorophores upon red excitation. Gold ZMWs increase emission of both fluorophores with Atto647N showing enhancement that is threefold higher than that observed for Atto565. This work indicates that 200 nm gold ZMWs are better suited for single-molecule fluorescence studies in the red region of the visible spectrum, while aluminum appears more suited for the green region of the visible spectrum.
AB - The effect of gold and aluminum zero-mode waveguides (ZMWs) on the brightness of immobilized single emitters was characterized by probing fluorophores that absorb in the green and red regions of the visible spectrum. Aluminum ZMWs enhance the emission of Atto565 fluorophores upon green excitation, but they do not enhance the emission of Atto647N fluorophores upon red excitation. Gold ZMWs increase emission of both fluorophores with Atto647N showing enhancement that is threefold higher than that observed for Atto565. This work indicates that 200 nm gold ZMWs are better suited for single-molecule fluorescence studies in the red region of the visible spectrum, while aluminum appears more suited for the green region of the visible spectrum.
UR - http://www.scopus.com/inward/record.url?scp=84985893580&partnerID=8YFLogxK
U2 - 10.1021/acs.jpca.6b03309
DO - 10.1021/acs.jpca.6b03309
M3 - Article
AN - SCOPUS:84985893580
SN - 1089-5639
VL - 120
SP - 6719
EP - 6727
JO - Journal of Physical Chemistry A
JF - Journal of Physical Chemistry A
IS - 34
ER -