A calorimetric, volumetric and combined SANS and SAXS study of hybrid siloxane phosphocholine bilayers

Mark B. Frampton; Doruntina Yakoub; John Katsaras; Paul M. Zelisko; Drew Marquardt

doi:10.1016/j.chemphyslip.2021.105149

A calorimetric, volumetric and combined SANS and SAXS study of hybrid siloxane phosphocholine bilayers

Mark B. Frampton, Doruntina Yakoub, John Katsaras, Paul M. Zelisko, Drew Marquardt

Labs & Soft Matter

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Siloxanes are molecules used extensively in commercial, industrial, and biomedical applications. The inclusion of short siloxane chains into phospholipids results in interesting physical properties, including the ability to form low polydispersity unilamellar vesicles. As such, hybrid siloxane phosphocholines (SiPCs) have been examined as a potential platform for the delivery of therapeutic agents. Using small angle X-ray and neutron scattering, vibrating tube densitometry, and differential scanning calorimetry, we studied four hybrid SiPCs bilayers. Lipid volume measurements for the different SiPCs compared well with those previously determined for polyunsaturated PCs. Furthermore, the different SiPC's membrane thicknesses increased monotonically with temperature and, for the most part, consistent with the behavior observed in unsaturated lipids such as, 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine, and the branched lipid 1,2-diphytanoyl-sn-glyerco-3-phosphocholine (DPhyPC).

Original language	English
Article number	105149
Journal	Chemistry and Physics of Lipids
Volume	241
DOIs	https://doi.org/10.1016/j.chemphyslip.2021.105149
State	Published - Nov 2021

Funding

SANS studies on Bio-SANS were supported by the OBER funded Center for Structural Molecular Biology (CSMB) under Contract FWP ERKP291, using the High Flux Isotope Reactor, a DOE Office of Science User Facility operated by the Oak Ridge National Laboratory (Oak Ridge, Tennessee, USA). JK is supported through the Scientific User Facilities Division of the Department of Energy (DOE) Office of Science, sponsored by the Basic Energy Science (BES) Program under Contract DEAC05–00OR22725. DM acknowledges support from the Natural Sciences and Engineering Research Council (NSERC). PMZ acknowledges support from the Natural Sciences and Engineering Research Council (NSERC) and the Advanced Biomanufacturing Centre (ABC) at Brock University.

Funders	Funder number
Advanced Biomanufacturing Centre
U.S. Department of Energy
Office of Science
Basic Energy Sciences	DEAC05–00OR22725
Natural Sciences and Engineering Research Council of Canada

Keywords

Lipid volume
Liposomes
Mixed chain lipids
Neutron scattering
Siloxane phospholipid
X-ray scattering

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10.1016/j.chemphyslip.2021.105149

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title = "A calorimetric, volumetric and combined SANS and SAXS study of hybrid siloxane phosphocholine bilayers",

abstract = "Siloxanes are molecules used extensively in commercial, industrial, and biomedical applications. The inclusion of short siloxane chains into phospholipids results in interesting physical properties, including the ability to form low polydispersity unilamellar vesicles. As such, hybrid siloxane phosphocholines (SiPCs) have been examined as a potential platform for the delivery of therapeutic agents. Using small angle X-ray and neutron scattering, vibrating tube densitometry, and differential scanning calorimetry, we studied four hybrid SiPCs bilayers. Lipid volume measurements for the different SiPCs compared well with those previously determined for polyunsaturated PCs. Furthermore, the different SiPC's membrane thicknesses increased monotonically with temperature and, for the most part, consistent with the behavior observed in unsaturated lipids such as, 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine, and the branched lipid 1,2-diphytanoyl-sn-glyerco-3-phosphocholine (DPhyPC).",

keywords = "Lipid volume, Liposomes, Mixed chain lipids, Neutron scattering, Siloxane phospholipid, X-ray scattering",

author = "Frampton, {Mark B.} and Doruntina Yakoub and John Katsaras and Zelisko, {Paul M.} and Drew Marquardt",

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doi = "10.1016/j.chemphyslip.2021.105149",

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AU - Frampton, Mark B.

AU - Yakoub, Doruntina

AU - Katsaras, John

AU - Zelisko, Paul M.

AU - Marquardt, Drew

PY - 2021/11

Y1 - 2021/11

N2 - Siloxanes are molecules used extensively in commercial, industrial, and biomedical applications. The inclusion of short siloxane chains into phospholipids results in interesting physical properties, including the ability to form low polydispersity unilamellar vesicles. As such, hybrid siloxane phosphocholines (SiPCs) have been examined as a potential platform for the delivery of therapeutic agents. Using small angle X-ray and neutron scattering, vibrating tube densitometry, and differential scanning calorimetry, we studied four hybrid SiPCs bilayers. Lipid volume measurements for the different SiPCs compared well with those previously determined for polyunsaturated PCs. Furthermore, the different SiPC's membrane thicknesses increased monotonically with temperature and, for the most part, consistent with the behavior observed in unsaturated lipids such as, 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine, and the branched lipid 1,2-diphytanoyl-sn-glyerco-3-phosphocholine (DPhyPC).

AB - Siloxanes are molecules used extensively in commercial, industrial, and biomedical applications. The inclusion of short siloxane chains into phospholipids results in interesting physical properties, including the ability to form low polydispersity unilamellar vesicles. As such, hybrid siloxane phosphocholines (SiPCs) have been examined as a potential platform for the delivery of therapeutic agents. Using small angle X-ray and neutron scattering, vibrating tube densitometry, and differential scanning calorimetry, we studied four hybrid SiPCs bilayers. Lipid volume measurements for the different SiPCs compared well with those previously determined for polyunsaturated PCs. Furthermore, the different SiPC's membrane thicknesses increased monotonically with temperature and, for the most part, consistent with the behavior observed in unsaturated lipids such as, 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine, and the branched lipid 1,2-diphytanoyl-sn-glyerco-3-phosphocholine (DPhyPC).

KW - Lipid volume

KW - Liposomes

KW - Mixed chain lipids

KW - Neutron scattering

KW - Siloxane phospholipid

KW - X-ray scattering

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A calorimetric, volumetric and combined SANS and SAXS study of hybrid siloxane phosphocholine bilayers

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