A biological standard for electron microprobe analysis of intracellular sodium concentration

V. S. Sottiurai, R. L. Malvin, L. F. Allard, W. C. Bigelow

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A method is described for the determination of the intracellular concentration of sodium in individual cells using the electron microprobe analyzer. This method gives an accuracy equal to that obtained by using flame photometry on tissues with large cell populations. Intracellular sodium was precipitated in the cell by a fixative containing pyroantimonate. Cartilagenous needles from shark fins which were equilibrated in saline solutions of differing concentrations were used as biological standards.

Original languageEnglish
Pages (from-to)749-751
Number of pages3
JournalJournal of Histochemistry and Cytochemistry
Volume24
Issue number6
DOIs
StatePublished - 1976
Externally publishedYes

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