α-Tocopherols location in membranes is not affected by their composition

Drew Marquardt; Norbert Kučerka; John Katsaras; Thad A. Harroun

doi:10.1021/la502605c

α-Tocopherols location in membranes is not affected by their composition

Drew Marquardt
, Norbert Kučerka
, John Katsaras
, Thad A. Harroun

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

To this day, α-tocopherols (aToc) role in humans is not well known. In previous studies, we have tried to connect aTocs biological function with its location in a lipid bilayer. In the present study, we have determined, by means of small-angle neutron diffraction, that not only is aTocs hydroxyl group located high in the membrane but its tail also resides far from the center of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers. In addition, we located aTocs hydroxyl group above the lipid backbone in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS), and sphingomyelin bilayers, suggesting that aTocs location near the lipid-water interface may be a universal property of vitamin E. In light of these data, how aToc efficiently terminates lipid hydroperoxy radicals at the membrane center remains an open question.

Original language	English
Pages (from-to)	4464-4472
Number of pages	9
Journal	Langmuir
Volume	31
Issue number	15
DOIs	https://doi.org/10.1021/la502605c
State	Published - Apr 21 2015

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title = "α-Tocopherols location in membranes is not affected by their composition",

abstract = "To this day, α-tocopherols (aToc) role in humans is not well known. In previous studies, we have tried to connect aTocs biological function with its location in a lipid bilayer. In the present study, we have determined, by means of small-angle neutron diffraction, that not only is aTocs hydroxyl group located high in the membrane but its tail also resides far from the center of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers. In addition, we located aTocs hydroxyl group above the lipid backbone in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS), and sphingomyelin bilayers, suggesting that aTocs location near the lipid-water interface may be a universal property of vitamin E. In light of these data, how aToc efficiently terminates lipid hydroperoxy radicals at the membrane center remains an open question.",

author = "Drew Marquardt and Norbert Ku{\v c}erka and John Katsaras and Harroun, \{Thad A.\}",

note = "Publisher Copyright: {\textcopyright} 2014 American Chemical Society.",

year = "2015",

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doi = "10.1021/la502605c",

language = "English",

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journal = "Langmuir",

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TY - JOUR

T1 - α-Tocopherols location in membranes is not affected by their composition

AU - Marquardt, Drew

AU - Kučerka, Norbert

AU - Katsaras, John

AU - Harroun, Thad A.

PY - 2015/4/21

Y1 - 2015/4/21

N2 - To this day, α-tocopherols (aToc) role in humans is not well known. In previous studies, we have tried to connect aTocs biological function with its location in a lipid bilayer. In the present study, we have determined, by means of small-angle neutron diffraction, that not only is aTocs hydroxyl group located high in the membrane but its tail also resides far from the center of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers. In addition, we located aTocs hydroxyl group above the lipid backbone in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS), and sphingomyelin bilayers, suggesting that aTocs location near the lipid-water interface may be a universal property of vitamin E. In light of these data, how aToc efficiently terminates lipid hydroperoxy radicals at the membrane center remains an open question.

AB - To this day, α-tocopherols (aToc) role in humans is not well known. In previous studies, we have tried to connect aTocs biological function with its location in a lipid bilayer. In the present study, we have determined, by means of small-angle neutron diffraction, that not only is aTocs hydroxyl group located high in the membrane but its tail also resides far from the center of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers. In addition, we located aTocs hydroxyl group above the lipid backbone in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS), and sphingomyelin bilayers, suggesting that aTocs location near the lipid-water interface may be a universal property of vitamin E. In light of these data, how aToc efficiently terminates lipid hydroperoxy radicals at the membrane center remains an open question.

UR - https://www.scopus.com/pages/publications/84928501055

U2 - 10.1021/la502605c

DO - 10.1021/la502605c

M3 - Article

C2 - 25317847

AN - SCOPUS:84928501055

SN - 0743-7463

VL - 31

SP - 4464

EP - 4472

JO - Langmuir

JF - Langmuir

IS - 15

ER -

α-Tocopherols location in membranes is not affected by their composition

Abstract

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