This data set contains N2O (nitrous oxide) efflux rates measured within SPRUCE experimental plots in 2024. N2O is a gaseous N species formed during the microbial processes of denitrification and ammonia oxidation, and is a powerful greenhouse. Measurements were made manually with a LiCor N2O/H2O analyzer (LI-7820) and paired SmartChamber (LI-8200-01S) in June, August, and October (2024-06-24 to 2024-10-22). During each measurement, the SmartChamber was placed on 8” PVC collars that were installed in May 2024. N2O flux was derived from 10-minute flux measurements processed using SoilFluxPro software (v5.3.1) and fit to a linear model. Model slope and R2 are reported along with soil water, soil temperature, and air temperature observations made with SmartChamber sensors. This dataset contains one data file in comma separate (*.csv) format.